TRANSFECTION OF HUMAN AIRWAY EPITHELIAL CELLS USING A LIPID- BASED VECTOR PREPARED BY THE HEATING METHOD

Summary The lung is an attractive organ for gene thera py due to the presence of cystic fibrosis and α 1 antitrypsin disorders whose genetic basis is known. However, low transfection efficiency, toxicity and immunogenicity issues have limited the success of the treatment strategies. In an attempt to overcome these limitations we formulated an anionic lipid-based vector using the heating method developed in our laboratory. The vector was characterised using atomic force microscopy and other techniques. The vector is completely non-toxic, possesses long term stability and is able to protect DNA from en zymatic degradation. The optimum ratio for transfection of human lung epithelial cells (16HBE14o-) was found to be 7:1 (lipid:DNA). These structures appear to be safe and effi cient in protection and delivery of DNA to the pulmonary tract.