Procedures and Modifications for Reliable Use of the Unidirectional Microflow Respirator in the Investigation of the Carotid Body of Gallus domesticus

Abstract An artificial respirator was used to ensure successful and precise artificial respiration in short and long term experiments during radical exposure of the caudal cervical region and thoracic cavity to investigate cytological changes in the carotid body of mature chickens (Gallus domesticus). Modification of the respirator to stabilize gas flow, temperature and humidity consisted of replacing the ball check valve in the heating and humidifying cylinder with a fine regulating stopcock in the water outlet of the cylinder and inserting eight glass rods in a circle inside the cylinder. Accumulation of water condensate in the trachea and lungs during long term experiments was prevented by inserting a cotton filter moisture trap in the tracheal cannula.