Sensitivity of ribosomal bound N-acetylphenylalanyl-tRNA to hydrolysis by a specific hydrolase

Abstract Binding of N- acetylphenylalanyl-tRNA , either enzymatically or non-enzymatically, to yeast 80-S ribosomes renders the substrate resistant to the hydrolytic action of a specific hydrolase present in yeast. In constrast, N- acetylphenylalanyl-tRNA bound to the 40-S ribosomal subunit is sensitive to enzymatic hydrolysis. The presence of the hydrolase in the aminoacyl-tRNA binding factor preparations greatly interferes with the formation of complexes between N- acetylphenylalanyl-tRNA and the small ribosomal subunit.