Method for filling gel chromatographic columns

The invention discloses a method for filling gel chromatographic columns. The method comprises the following steps: preparing uniform slurry, namely adding gel powder into deionized water, fully wetting, then heating and boiling for 2-3 hours so as to obtain the uniform slurry; (2) connecting a filling system, namely connecting the upper end of a hollow column tube onto a uniform-slurry tank, and connecting a stainless-steel capillary tube with the inner diameter being 0.2-0.6mm with the lower end of the hollow column tube; (3) filling the deionized water with the volume being about 1/3 of the volume of the hollow column tube, and then slowly adding the uniform slurry into the uniform slurry tank; (4) starting an air compressor, pressing into displacing liquid, and maintaining for 20-180 minutes under the pressure of 5.5-8.5MPa, wherein the displacing liquid is a mixture of water and methyl alcohol; and (5) washing a column bed with the deionized water as displacing liquid with the amount being 1-3 times of the volume of the column, so as to prepare the gel chromatographic columns. The method disclosed by the invention has the advantages that according to the gel chromatographic columns prepared by using the method, bubbles in fillers can be eliminated, and the column bed can be closely filled with the fillers, so that the efficiency of the chromatographic columns is obviously improved, and simultaneously, the success rate for filling the chromatographic columns is increased from 76% to more than 90%.