일회성 운동 및 회복기 흰쥐 골격근 내 HSP27과 관련된 Atypical MAPK 발현 경향

This study was to investigate the effects of Atypical MAPK signaling pathway expression on HSP27 expression which was small heat shock protein (HSP) related on damage and recovery of skeletal muscle. Generally, acute or high intensive exercise can elicit skeletal muscle injuries, cellular inflammatory response. Mitogen activated protein kinase (MAPK) highly involves exercise induced cell damage and recovery which have regulatory function on small HSP expression. MAPK is stimulated by stress and reative oxygen species induced by exercise. As a expect, MAPK may stimulates HSP27 which has protectional function of skeletal muscle cytoskeleton and is involved in protein synthesis and recovery. However exact regulatory mechanism between MAPK and HSP 27 not elucidated. To investigate the relation and regulatory function between Atypical MAPK and HSP27 during exercise and recovery, we did acute exercise and examined protein expression in gastrocnemius of ICR mouses by western blotting. Muscle harvest was various which divided by 5 time point (exercise: 15mins, 60mins / recovery: 6hours, 12hours), and control groups. As a result, the proteins expression of MAPK sub factors, PAK2, ERK3, and MK5, showed similar tendency to aspects of HSP27 revelation during exercise and recovery period. However, PAK1 was seen an inverse tendency different from other MAPK sub factors during exercise and recovery period. PAK1, in general, was seen a similar tendency to HEK293, PC12 cell, and had a same tendency to the result of preceding studies on the importance of signaling pathway to maintain the cell homeostasis. Thus, Atypical MAPK signaling pathway was important to prevent from skeletal muscle injuries caused by exercise stress.