Effects of 4,4'-Diisothiocyanato-stilbene-2,2'-disulfonic Acid (DIDS) and Chlorpromazine on NO3− Transport via Anion Exchanger in Erythrocytes: Inertness of DIDS in Whole Blood

We examined the effects of chlorpromazine on NO3− transport between erythrocytes (RBCs) and extracellular fluid. Chlorpromazine (10 μg/ml) did not influence NO3− movement in both whole blood and RBC suspension. Though an anion exchanger (AE1) inhibitor DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid, 100 μM) did not alter NO3− movement in whole blood, it inhibited the movement in a concentration-dependent manner in the RBC suspension. The inhibition was abrogated by plasma and albumin concentration-dependently. Our results indicated that chlorpromazine had no effect on NO3− transport through AE1 and that the inertness of DIDS on AE1 in whole blood is due to interference by albumin in plasma.