Changes of HSP70,HSP70 mRNA,SOD,NOS in uterus of natural climacteric rats and the intervention of preventive moxibustion on Guanyuan point

Objective:To investigate the influence and mechanism of moxibustion on the acupoint of Guanyuan (CV4) on HSP70,HSP70 mRNA,SOD,NOS in uterus of natural climacterium rats. Methods: 112 ten month-aged SD female rats examined by vaginal smear were chosen and divided into seven groups. And another 16 three and half month-aged SD female rats were absorbed. Immunohistochemical, immunoturbidimetric assay and hybridiztion in situ and the like methods were applied to observe the effect on HSP70, HSP70 mRNA, SOD, NOS in uterus of 12, 14 and 16 month–aged menopausal rats with preventive accupuncture on Guanyuan point(twice a week,eight weeks continued) in 10th month. Results: In normal group, the expression of HSP70 and HSP70 mRNA in uterus increased at the beginning and descended in the end with the month growing, the activity of SOD, NOS decreased significantly(P0.01). Compared with control group at the same month-old condition, the expression of HSP70 and HSP70 mRNA of preventiver accupunctre group increased obviously(P0.05 or P0.01), and the activity of SOD and NOS increased. Conclusion: Preventive accupuncture on Guanyuan point can improve the expression of HSP70 and HSP70 mRNA and the activity of SOD and NOS in uterus. It might be one of mechanisms of protecting uterus with preventive moxibustion.