Molecular analysis and clinicopathologic features of advanced colorectal cancer in Algerian patients

Aims: this retrospective study aims to analyze tumors hot spot mutations frequency in KrAs, brAF and microsatellite instability (MsI) status of tumors in Algerian patients with advanced colorectal cancer (crc) which can predict prognosis and contribute to decisions on treatment strategies. Methods: KrAs exon 2, brAF exon 15 were analyzed by direct sequencing of amplified Pcr products in 102 tumors patients with advanced crc cancer. the MsI was determined using a panel of five mononucleotide markers (bAt25, bAt26, Nr21, Nr22 and Nr24). results: brAF and KrAs mutations were detected in 4.9% and 31.3% of the tumors