Effect of pyrazoles on mitochondrial bioenergetics in cardiovascular cells

Oxidative stress is the overproduction of reactive oxygen species by the mitochondria. This byproduct can lead to a variety of cardiovascular diseases as it causes apoptosis. Antioxidants are commonly used in medicine to relieve such symptoms. To study the effects of pyrazoles on the of oxidative stress in the myocardium, fibroblast cells from a C57 black wild type male mouse heart were cultured and oxidative stressed was induced. They were then treated with pyrazoles KT-01-15D, KT-01-17F, KT-01-19A. The oxygen consumption rate and extracellular acidification rate were then tested using the Agilent Seahorse XF24 analyzer. The pyrazoles were found to have a significant impact on the mitochondrial function of the cells. They successfully healed the cell of the reactive oxygen species that interrupted the processes of cellular respiration. Our findings suggest that pyrazoles KT-01-15D, KT-01-17F, KT-01-19A play an important role in the recovery of normal function of cardiomyocytes.