Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro

The hemagglutinating virus of Japan (HVJ; also called the Sendai virus) envelope has been developed as a safe and efficient non-viral vector. Because replication and transcription of genomic RNA is inactivated by β-propiolactone treatment or UV-irradiation, the HVJ envelope is extremely safe. This unit describes the method of transfection of siRNA and protein with the HVJ envelope. Curr. Protoc. Immunol. 89:10.17D.1-10.17D.9. © 2010 by John Wiley & Sons, Inc. Keywords: hemagglutinating virus of Japan; Sendai virus; HVJ envelope; siRNA transfection