Prenylflavonoids counteract ovariectomy-induced disturbances in rats

Abstract The aim of the study was to investigate in animal model the influence of hops prenylflavonoids on hot flashes and bone loss. Xanthohumol (XN) and hop prenylflavonoids extract (HOP) restored twenty-four-hour variability of tail skin temperature in ovariectomized rats. Additionally XN and HOP increased femoral bone mineral density (BMD) (0.268 ± 0.019 g/cm2 and 0.256 ± 0.013 g/cm2 vs. 0.214 ± 0.015 g/cm2). In micro-computed tomography (mCT) XN and HOP led to a thickening of trabeculae in femurs and vertebral bodies. HOP increased serum osteocalcin (OC) (222.86 ± 39.62 pg/mL vs. 175.87 ± 58.91 pg/mL) and decreased tartrate-resistant acid phosphatase (TRACP) (0.714 ± 0.132 U/L vs. 1.251 ± 0.304 U/L), whereas in XN only decreased TRACP (0.738 ± 0.178 U/L). Results of our study showed that HOP and XN ameliorate ovariectomy induced disturbances in tail skin temperature (TST). Additionally, HOP exerts a mild protective effect on bone loss induced by decline in oestrogen level.