Reagents, chaotropic agents, kits and methods for isolating nucleic acids based on magnetic materials cellulose

A method for isolating DNA from a sample by promoting the binding of DNA contained in the sample with a material magnetic cellulose, the method characterized by having the steps of: (1) providing a material magnetic cellulose and a reagent comprising: a chaotropic solution of a univalent selected group metal salt consisting of lithium, sodium acetate and sodium chloride, and an alcohol selected from the group consisting of ethanol, isopropanol and a mixture selected arbitrarily ethanol and isopropanol chloride in univalent metal salt that has a concentration ranging from about 0.05 to 2.0M, and alcohol having a concentration ranging from about 10% to 80% by volume, and the pH of the chaotropic solution varies about 5.0 to 7.5; (2) adding the reagent and cellulose material magnetic sample containing DNA and a plurality of substances which are not nucleic acids; and mixing the reagent, the material magnetic cellulose and the sample, allowing the chaotropic solution of said reagent to break hydrogen bonds, the interactions of Van der Waals, or hydrophobic effects between DNA and the environment in which is located the nucleic acid of the sample, so that the magnetic material and transport one cellulose DNA; and (3) eluting the DNA from cellulose magnetic material.