AUGMENTATION OF BIOREMEDIATION ABILITY BY EXOGENOUS BACTERIA IN RHIZOSPHERE OF CORCHORUS CAPSULARI

Two fusants, Tu-An and Tu-P, were obtained through the protoplast electrofusion of the EGFP labeled bacteria(An,the highly-effective anthracene-degrading bacteria,and P,the biosurfactant-producing bacteria), and the dominant bacteria in Jute rhizospheric soil, the two fusants and their starting strains were colonized in soil cultivated with jute(Corchorus capsulari),and polluted by anthracene. The colonization of bacteria,plant growth and anthracene degradation were tested at different pollutant concentrations.The experimental results showed that the fusants in rhizosphere had much higher population density than the starting strains in the same amount of inoculation;when the two fusants coexisted they also had slightly higher population density than the single fusant; when the exogenous bacteria were added in soil, plant obtained more favourable growth condition and could grow well,and in soil without adding degrading bacteria,the growth of jute was oppressed seriously, indicating that the inoculation of the exogenous bacteria into contaminated soil could stimulate biodegradation and achieve bioaugementation. The result reveals that it is feasible to make remediation by bioaugmentation through adding exogenous bacteria into contaminated soil. Fig 3 , Tab 2, Ref 20