Microsatellite mapping of genes for branched spike and soft glumes in Triticum monococcum L.

The locus responsible for branched spike in a branched spike mutant of Triticum monococcum L. (2n = 2x = 14, AmAm genome) and soft glume in Triticum sinskajae Filat. et Kurkiev (2n = 2x = 14, AmAm genome) were mapped by genotyping F2 populations using microsatellite markers. Phenotypic analysis in the cross T. sinskajae PI 418587/a branched spike mutant KT3-24 confirmed that both characters were under control of a recessive allele at a single locus, and they were linked with 26.6 cM. The branched head in T. monococcum (bh m ) locus was located on chromosome 2AmS and the marker Xgwm122 flanked the bh m gene distally. Soft glume locus in T. sinskajae was allelic to the soft glume (sog) locus in mm09, a soft glume mutant of T. monococcum. The sog locus was linked with Xwmc644 distally. In the F2 hybrids of T. monococcum #252/PI 418587 and T. monococcum KT 3-21/PI 418587, sog was linked with Xgwm71. The gene fg which determines a false glume was also located on chromosome 2AmS and the recombination between sog and fg (1.6 cM) was obtained in F2 hybrid of KT 3-21/PI 418587.