ACCEPTED MANUSCRIPT Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma

�In the present study, we aimed to examine whether SET domain-containing methyltransferases are upregulated in different classes of renal cell carcinoma. We immunoblotted against SET domain and quantitated the expression of these modular domains. Furthermore, we examined the expression of Rad51, the key protein that confers genomic stability. There was enhanced expression of SET domain-containing histone methyltransferases in whole lysates of all classes of renal carcinoma. In metastatic high grade clear cell carcinoma, this expression was more pronounced. Though we could not demonstrate direct correlation, we showed that epigenetic modification by methylation is associated with decreased genomic translation of Rad51.