microRNA-342-5p and miR-608 inhibit colon cancer tumorigenesis by targeting NAA10

// Hongju Yang 1,* , Qian Li 2,* , Jie Niu 3,* , Bai Li 2 , Dejun Jiang 1 Zhihua Wan 1 , Qingmei Yang 1 , Fei Jiang 1 , Ping Wei 1 and Song Bai 1 1 The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China 2 Human Genetics Center of Yunnan University, Kunming, Yunnan, China 3 Institute of Medicinal Biology Chinese Academy of Medical Science, Kunming, Yunnan, China * These authors have contributed equally to this work Correspondence to: Song Bai, email: // Keywords : NAA10, miR-342-5p, miR-608, colon cancer Received : June 03, 2015 Accepted : October 14, 2015 Published : December 04, 2015 Abstract miRNAs have been shown to play pivotal roles in the establishment and progression of colon cancer, but their underlying mechanisms are not fully understood. N-acetyltransferase NAA10 participates in many cellular processes, including tumorigenesis. Here we showed that miR-342-5p and miR-608 suppressed the tumorigenesis of colon cancer cells in vitro and in vivo by targeting NAA10 mRNA for degradation. Overexpression of miR-342-5p or miR-608 decreased NAA10 mRNA and protein levels and thereby suppressed cell proliferation, migration, and cell-cycle progression, as well as promoted apoptosis in SW480 and SW620 cells. More importantly, miR-342-5p and miR-608 significantly decreased the tumorigenic capacity of SW480 and SW620 cells in a mouse xenograft model. We also observed an inverse correlation between the expression of NAA10 and that of both miRNAs. Our results implicate miR-342-5p and miR-608 in colon cancer development and unveil the underlying mechanism of this phenomenon, which involves NAA10.