Effect of cigarette smoking on DNA damage of human cumulus cells analyzed by comet assay

Abstract Cigarette smoking has been reported to induce intrafollicular oxidative stress that may lead to DNA damage. The purpose of this study was to determine damage in DNA in human cumulus cells caused by tobacco smoke in females who had received in vitro fertilization. The level of DNA damage in freshly isolated cumulus cells was determined by comet assay. Statistically significant increase ( p