STAINING OF HISTOLOGICAL SECTIONS FROM THE SMALL INTESTINE USING HIBISCUS SABDARIFFA

Dids Link : http://dids.info/didslink/09.2014-87771635/ ABSTRACT Objective: this was a descriptive study aimed to assess the quality of staining of intestinal tissue by Hibiscus Sabdariffa solution compared to Hematoxylin-Eosin routine stain. Methods: paraffin-embedded formalin-fixed tissue sections from small intestine were stained by Hibiscus Sabdariffa solution using different concentrations and time durations in room temperature. Results: With 5 % solution, 48 % of slides showed at least very good quality of staining. The excellent results were mainly obtained when time duration was 60 minutes while poorest results were obtained when staining time was 1-2 minutes Conclusion: Hibiscus Sabdariffa solution is a cheap, natural, and harmless dye that can be used for staining of histological sections and it is comparable to the routine Hematoxylin and Eosin stain.