Prediction of ovarian malignancy using plasma cell-free DNA

Objective Evaluation of cell-free DNA (cf-DNA) as a noninvasive tool to discriminate between malignant and benign ovarian growth. Patients and methods DNA was extracted from the plasma of 50 women with ovarian lesion. A total of 20 women with pathologically proven malignancy and 30 women with benign ovarian lesions were included. Quantification of cf-DNA was performed using real-time PCR. cf-DNA was correlated with the histopathology. Results The preoperative plasma cf-DNA level was statistically significantly higher (P Conclusion Detection of cf-DNA holds promise as a diagnostic test for women with ovarian cancer. We recommend its incorporation into the workup investigations for women attending with adnexal mass.