Cloning and sequence analysis of a S-adenosyl-L-homocysteine hydrolase gene from Volvariella volvacea.

Mating experiments involving either monokaryotic basidiospores or protoplast monokaryons derived from Pleurotus eryngii parental strains No.6 and ACCC50894 were used to generate 65 and 37 hybrid strains,respectively.Fruiting tests were carried out on hybrids selected on the basis of mycelial growth rate and growth vigor,and fruit bodies were successfully obtained from 15 hybrids.Grey correlation analysis based on a comprehensive evaluation of seven selected agronomic traits revealed that hybrids D12,D18,D20,D30,D19,D31,D17 and D48 obtained from monospore crossings were superior to the two parental strains,while hybrids Y29 and Y30 obtained from protoplast monokaryon crossings ranked between the two parental strains.