Dendritic cells transduced with lentiviral vector targeting RelB gene using RNA interference induce hyporesponsiveness in memory CD4+ T cells and naïve CD4+ T cells

Abstract The ability of DCs to induce immune tolerance depends on its maturation status. RelB plays a pivotal role in DCs differentiation. A therapeutic protocol of DCs-based not only induces hyporesponsiveness in T N s, but also in alloreactive T M s is required. Thus, it is urgent to assess modulatory effects of RelB-silenced DCs on T M s and T N s. In this study, we constructed lentiviral vector which could efficiently silenced the RelB in DCs (DCs-miR RelB) to keep them immature. These DCs induced antigen-specific hyporesponsiveness in CD4 + T N s. In contrast, upon re-stimulation with mature DCs, CD4 + T M s primed by DCs-miR RelB maintained hyporesponsiveness in terms of proliferation and cytokine production. And these may be associated with micro155 and micro181a expression levels in T M s and T N s. These results may help developing the DCs-based therapeutical protocols by inducing hyporesponsiveness in CD4 + T N s and T M s.