Isolation and Functional Analysis of Human Neutrophils.

This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described. © 2015 by John Wiley & Sons, Inc. Keywords: neutrophil; leukocyte; NOX2; phagocyte; innate immunity