Water jet indentation for local elasticity measurements of soft materials

We present a novel elastography method for soft materials (100 Pa-100 kPa) based on inden- tation by a μm-sized water jet. We show that the jet creates a localized deformation ("cavity") of the material that can be easily visualized. We study experimentally how cavity width and depth depend on jet speed, height, incidence angle and sample elasticity. We describe how to calibrate the indenter using gels of known stiffness. We then demonstrate that the indenter yields quantitative elasticity values within 10% of those measured by shear rheometry. We corroborate our experimental findings with fluid-solid finite-element simulations that quantitatively predict the cavity profile and fluid flow lines. The water jet indenter permits in situ local stiffness measurements of 2D or 3D gels used for cell culture in physiological buffer, is able to assess stiffness heterogeneities with a lateral resolution in the range 50-500 μm (at the tissue scale) and can be assembled at low cost with standard material from a biology laboratory. We there- fore believe it will become a valuable method to measure the stiffness of a wide range of soft, synthetic or biological materials.