Systematic Analyses on Mouse Genome Encyclopedia

Our Mouse Encyclopedia Project, which performs exhaustive collection and sequencing of all mRNA species expressed in mouse, successfully collected more than 70,000 different kind of cDNA clones classifiable by 3’-end sequence tags. By Using those clones, we are completing sequence of the isolated cDNA clones in concurrent manner, which piled up to over 10,000 sequences. This number tells that our project reached to the new realm where other genome project could not have reached in terms of the total number of genes sequenced. In contract to the genomic sequencing, our strategy choosing cDNA as the sequencing material brought us big advantages that the amount of sequencing required to obtain all gene information requires much less effort in terms of the number sequence runs, and each gene information are ready for application, upon completion of individual sequence with out further artificial processing of data, i.e. prediction of gene region and exon structure. By taking this advantage, we have started several projects such as analyses of expression profiles and proteinprotein interaction. In the current study, we report results of one of such application, produced by bioinformatic approaches.