Additional file 2: Figure S1. of “Hit-and-Run” transcription: de novo transcription initiated by a transient bZIP1 “hit” persists after the “run”

Experimental design. (A) Vector maps of the pBeaconRFP construct containing 35S::GR::bZIP1 [6, 15] and the pJD385 empty vector (EV) control containing a 35S::GR cassette. (B) A 20 minute exposure to 4tU was sufficient to detect significant incorporation in RNA fractions of Arabidopsis cells, as shown by dot blot imaging of 1 μg RNA from 4.5 106 cells exposed to 1.5 mM 4tU (+) or uracil only (−). (PDF 36 kb)