LANDSCAPE PHAGE LIBRARIES AS A SOURCE OF BIOSELECTIVE NANOMATERIALS

New generations of targeted diagnostic, th erapeutic and imaging nanodevices require robust molecular recognition interfaces that bind target receptors with high specificity and selectivity. Landscape phages are nanoparticles displaying on their surface thousands of specific ligands. We constructed a novel landscape library, in which random 9-mer peptides are presented on the phage surface in 4000 copies. The new library and previously constructed 8-mer library have been used to select phages that bind proteins (streptavidin, TEM1 βlactamase) and pathogenic bacteria S. typhimurium. Phage-borne peptides selected from two libraries mostly have no overlapping or common motifs indicating that the libraries have distinct repertoires of binding phages. Selected phages were used as recognition interfaces in phage-based microarrays and biosensors. Constructed landscape phage libraries can serve as an universal source for novel stable bioselective nanomaterials with many applications in medicine and technology.