Loss of epidermal growth factor receptor on the renal neoplasm induced in vivo with xenotropic pseudotype Kirsten murine sarcoma virus

Summary A characteristic feature of normal rat kidney (NRK) cells in their ability to bind epidermal growth factor (EGF) and loss of the ability to bind EGF in the renal neoplasm induced in vivo with C type RNA virus were demonstrated. Maximal binding capacity and apparent dissociation constant of NRK cells were 3.36 femtomoles of EGF bound per 10 6 cells and 2.85 × 10 −11 M, respectively. The binding of EGF to transformed cell in vivo with Xenotropic pseudotype Kirsten murine sarcoma virus was abolished.