Development of an ultra sensitive LC-MS/MS method for the simultaneous determination of Δ9- Tetrahydrocannabinol (THC) and one of its major metabolites Δ9-(11-OH) THC in human plasma.

n Mean (pg/mL) Accuracy (%) Bias (%) Intra CV (%) Total CV (%) LLOQ 10.0 pg/mL 18 9.33 93.3 -6.7 4.2 4.5 Low 30.0 pg/mL 18 28.5 95.1 -4.9 3.2 3.3 Medium 200 pg/mL 18 197 98.6 -1.4 2.0 2.7 High 4000 pg/mL 18 3720 93.0 -7.0 1.9 2.0 OQC 1 40000 pg/mL 18 39000 97.6 -2.4 1.8 2.5 11-OH-THC n Mean (pg/mL) Accuracy (%) Bias (%) Intra CV (%) Total CV (%) LLOQ 10.0 pg/mL 18 10.0 100.0 0.0 5.0 6.8 Low 30.0 pg/mL 18 29.8 99.4 -0.6 3.6 3.7 Medium 200 pg/mL 18 199 99.5 -0.5 2.3 2.5 High 4000 pg/mL 18 3730 93.3 -6.7 1.3 1.7