Transfer and bioavailability of inorganic and organic arsenic in sediment-water-biota microcosm

Abstract Arsenic (As) contamination in the sediments has received increasing attention, but its transfer and bioavailability to benthic infauna remain much less well known. In the present study, we deployed the diffusive gradients in thin films (DGT) to quantify the different As speciation in the overlying water and porewater, and assessed the exposure pathway, transfer and bioavailability of different As species in an infaunal clam Sinonovacula constricta. We demonstrated a very dynamic transformation and exposure of As in the sediment-water-clam microcosm. In such microcosm, arsenite [As(III)] in the overlying water, pore water and sediments was almost oxidized to arsenate [As(V)]. Conversely, the accumulated As(V) in the clams was reduced to As(III), followed by methylation to dimethylarsinic acid (DMA), whereas the overall conversion of toxic inorganic As species to less-toxic arsenobetaine (AsB) was much poor in the clams. Moreover, biotransformation depended on the As accumulation level. As(III) was the predominant As species in the control and the Low As treatment clam, whereas DMA was the predominant As species in the High As treatment clam. Significant and positive correlations were found between As(V) concentrations in the clams and those in DGT-labile As in overlying water/pore water, as well as between the DMA and As(V) concentrations in the clams and those in the sediment. DMA and As(V) in the sediments was much more bioavailable to the clams than inorganic As [As(III)] and AsB. Moreover, As(III) and As(V) in the overlying water and pore water, as well as DMA and As(V) in the sediments displayed high migration ability. Coupled DGT technology and biotransformation study therefore suggested that metabolism of ingested As species as well as ingestion and retention of DMA resulted in high DMA bioaccumulation in clams.