Comparative studies on the molecular genetic diversities between the Zhoushan wild Miichthys miiuy and their early filial generation by AFLP markers

Genetic diversity of a wild population and an artificial breeding population of Miichthys miiuy was investigated with amplified fragment length polymorphism(AFLP).The wild population was caught with cast net in the East China Sea near Zhoushan,and the cultured population was artificial breeding offspring of the wild population which was the first filial generation(F1).Eight EcoR I and Mse I primers combination produced 505 clear bands,of which 369 bands were polymorphic loci.The overall proportion of polymorphic loci was 73.07%.The two groups of parental and offspring generation's polymorphic loci,percentage of polymorphic loci,Nei genetic diversity index and Shannon information diversity index were 367,349,72.51%,68.86%,0.422 2,0.413 9 respectively.The coefficients of genetic similarity of the parental and the offspring generation were 0.987 6,and 0.971 5.Miichthys miiuy parent's population,Nei index of genetic diversity,genetic similarity and the Shannon information diversity index were higher than those of the offspring population.The genetic similarity coefficient and genetic distances between two populations were 0.979 6,and 0.020 6.Gst coefficient of gene differentiation was 0.022 3.There was no significant genetic differentiation between two populations.The analysis of molecular variance(AMOVA) results showed 97.78% of the genetic variation among individuals within groups.There was no significant genetic variation between two populations.