Analysis of whole genome of 25 novel coronaviruses in Jiangsu province/ 江苏省25株新型冠状病毒全基因组分析

Objective The entire genome sequences of 25 novel coronaviruses in Jiangsu province were analyzed and their evolutionary characteristics were studied Methods High-throughput sequencing was used to sequence the throat swab samples from confirmed cases Single nucleotide polymorphisms were analyzed using CLC Genomics Workbench 12 0 software Evolution characteristics were analyzed by MEGA 5 1 Results A total of 52 single-base substitution mutations were detected in 25 strains Phylogenetic analysis showed 25 stains were clustered into two clades Viruses in clade 1 contain 8682 and 28144 CT SNP links While viruses in clade 2 contain mutations in those two bases, i e , 8682 (ORF1ab: C8517T, synonymous mutation) and 28144 (ORF8: T251C, L84S) Among clade 2, five stains subclustered into one group based on SNP links in 24034 (S: C2472T, synonymous mutation), 26729 (M: T207C, synonymous mutation), and 28077 (ORF8: G184C, V62L) There were no significant differences in the distribution of different clades/subclusters in the population and the disease types Conclusions We have found some SNPs occurred in new coronaviruses The effects of different SNPs on virus transmission and pathogenicity need to be further studied