Caracterização morfológica e imuno-histoquímica de lesões em casos de aborto bovino bacteriano e viral no

Orlando D.R., Costa R.C., Abreu R.V.S., Abreu C.C., Nakagaki K.Y.R., Wouters A.T.B., Raymundo D.L. & Varaschin M.S. 2014. (Morphological and immunohistochemi- cal characterization of the lesions in cases of bacterial and viral bovine abortion in southern Minas Gerais, Brazil.) Caracterizacao morfologica e imuno-histoquimica de le- soes em casos de aborto bovino bacteriano e viral no sul de Minas Gerais. Pesquisa Veteri- naria Brasileira 34(10):974-980. Setor de Patologia Veterinaria, Universidade Federal de Lavras, Cx. Postal 3037, Lavras, MG 37200-000, Brazil. E-mail: msvaraschin@dmv.ufla.br The paper evaluates the participation of bacterial and viral agents in bovine abortions in farms of southern Minas Gerais state, Brazil. Histopathological and immunohistochemi- cal analysis was performed in aborted fetuses of cattle received by the Setor de Patologia Veterinaria, Universidade Federal de Lavras, from 1999 to 2013. From 60 fetuses studied, 30 (50%) had microscopic lesions. From these, eight had lesions consistent with bacterial agents and three had lesions suggestive of viral agents. In the bacterial abortions, one fe- tus presented lesions compatible with leptospirosis, characterized by jaundice, cholestasis, lymphoplasmacytic intersticial nephritis, and tubular nephrosis. Seven fetuses had puru- lent pneumonia or bronchopneumonia and one of them had also fibrinous pleuritis and peritonitis; two of them presented positive immunostaining for Brucella abortus. One of the three fetuses with lesions of viral infection revealed positive imunostaining for bovine herpesvirus. The results showed that abortions of bacterial and viral origin occur in the Region of this study and prophylactic measures should be adopted on the farms. This study also demonstrates that immunohistochemistry associated with histopathology is a useful and viable tool for the diagnosis, especially when microbiological and/or serological tests are not available.