Electroantennogram reaction test method of corn borer female silkworm to corn plant volatile substances
2014
The invention discloses an electroantennogram reaction test method of corn borer female silkworm to corn plant volatile substances. The method comprises the steps of feeding asiatic corn borer by an artificial feed in an artificial climatic box, continuously breeding multiple generation, at the day when adults are subjected to eclosion, freely copulating the male worm and the female worm in a breeding cage according to the proportion which is not less than 2:1, feeding 10% hydromel to supply nourishment, and taking the female silkworms which copulate after 3 days as a experimental worm resource for test; selecting 20 kinds of corn volatile substance standard chemical compounds, dissolving each sample into liquid paraffin, adequately mixing in a vortex mixer, and preparing 5*10 g/ml of solution for EAG test; and carrying out electroantennogram test on the worm resource for test. The method shows that the corn borer has different EAC reaction values to the different volatile matter components of the corn, and illustrates that the osmoceptor of the asiatic corn borer has different sensibility and selectivity to the smell from the host habitat, so that the basis for researching the oviposition attractant or repellent of the asiatic corn borer can be provided, and the novel pest control means can be explored.
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