Solubilization and partial characterization of adenosine binding sites from rat brainstem.
1983
Abstract Binding sites for adenosine were solubilized from rat brainstem membranes with either sodium cholate, sodium deoxycholate or 3-[3-cholamidopropyl)dimethyl-ammonio]-1-propanesulfonate. About 30% of the binding activity were released by these detergents as assayed by [ 3 H]phenylisopropyladenosine (PIA) binding. Specific [ 3 H]PIA binding to the solubilized fraction was saturable and was found to be a monophasic saturation profile. In contrast, [ 3 H]PIA binding sites. By gel filtration on a Sepharose CL-6B biphasic profile suggesting the presence of two binding sites. By gel filtration on a Sepharose CL-6B column, the adenosine binding site—detergent complex was estimated to have app. M r 280 000 and r s = 5.4 nm.
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