P15(上标 INK4B)基因对人胰腺癌细胞系BxPC3增殖的影响

Objective To investigate the effect of p15(superscript INK4B) (p15) gene transfection on the proliferation of pancreatic cancer cell line BxPC3. Methods In pl5 transfection group, pCDNA3.1 (+) p15 was transfected into BxPC3 cell by the vector of Lipofectamine2000. In empty plasmid transfection group, pCDNA3.1 (+) neo was transfected into BxPC3 cell with the same method as a blank control group. In non transfection group, the BxPC3 cell was not transfected as a negative control group. The p15 mRNA expressions were assayed by RT-PCR, and p15 protein expressions were assayed by Western blot. The proliferation was determined by MTT assay, ultra-structure changes were measured by transmission electron microscope. Cell cycle and apoptosis were measured by flow cytometry. Results In the pCDNA3.1 (+) p15 transfection group, the expression of p15 mRNA and protein were resumed. Since the 2nd day of culture, the growth of pCDNA3.1 (+) p15 transfection group was inhibited, till the 7th day, the inhibitory rate was 47.9% , phase cell accounted for (61.56 ± 3.96) % of all the cells, which was significantly higher than (47.44±6.35) % of the black control groups and (49.22 ± 7.23) % of the negative control group (P＜0.05). apoptosis peak occurred and the apoptosis rate was (5.27 ± 1.04)% in pCDNA3.1 (+) p15 transfection group, which was significantly higher than (0.11 ± 0.06) % of the black control groups and (0.09 ± 0.07) % of the negative control group (P＜0.05). Apoptosis was also observed by transmission electron microscope in the pCDNA3.1 (+) -p15 transfection group cells. Conclusions After p15 gene transfection, BxPC3 cell proliferation could be significantly inhibited and apoptosis could be induced.