Tissue culture effect of different size explants of Lycoris aurea

Objective :To solve the puzzle about the right size of the explant of wild medicinal plant Lycoris aurea for tissue culture. Methods :three-size explants:9.0×8.0 mm、6.0×5.0 mm、3.0×2.0 mm of endothecium bulb-scale joined by a strip of stem plate and middle-layer bulb-scale joined by a strip of stem plate were cultured on two kinds of the medium:MS+9 mg/L BA +5 mg/L NAA+0.7% agar +3% cane sugar and MS+5 mg/L BA +9 mg/L NAA+0.7% agar +3% cane sugar,and the culture effects of the three-size explants for Lycoris aurea were studied.Results and Conclusion :The results showed that the explant size on 6.0×5.0 mm of the endothecium bulb-scale cultured on the medium:MS+9 mg/L BA +5 mg/L NAA+0.7% agar +3% cane sugar had the advantage of forming adventitious buds and roots,and inhibiting brown samples,and to the middle-layer bulb-scale explant of the same size cultured on the same medium was next. Correlation analysis showed that the culture effect of the three-size explants on one kind of medium was significantly correlated with that of theirs on another kind of medium,which further proved that the result of Duncan's multiple range test,i.e. if the effect of an explant size on one kind of medium were better,its effect on another kind of medium would also be better. Both Duncan's multiple range test and correlation analysis indicated that the effect of the size on 6.0×5.0 mm explant of the double endothecium bulb-scale joined by a strip of stem plate was better than those of the other sizes on the experimental explants,and to that of 6.0×5.0 mm size of the double middle-layer bulb-scale joined by a strip of stem plate was next.