Validação de marcadores moleculares associados à resistência à ferrugem marrom em cana-de-açúcar

ABSTRACT Brown rust caused by the fungus Puccinia melanocephala is an important disease affecting sugarcane ( Saccharum spp.) and is present in almost all growing areas. A major effect gene, Bru 1, was described as a durable resistance source in sugarcane against isolates of P. melanocephala . The aim of this study was to evaluate the efficiency of two molecular markers strongly associated with Bru 1 gene, R12H16 and 9O20-F4-RsaI, for prediction of genetic resistance to brown rust in sugarcane varieties. Thus, this study included 14 RB varieties, developed by RIDESA (Interuniversity Network for the Development of the Sugarcane Industry), which were among the 10 most cultivated varieties in the central-south region of Brazil in the period of 1974-2015. To evaluate the disease in the field an experiment was conducted in randomized blocks with four replicates. The severity of Barreto, F.Z.; Balsalobre, T.W.A. Chapola, R.G.; Hoffmann, H.P.; Carneiro, M.S. Validation of molecular markers associated with brown rust resistance in sugarcane.