Improved enantiomeric separation with a 2,6-di-O-pentyl-3-O-trifluoroacetylated β-cyclodextrin and OV-7 mixed stationary phase chiral capillary column

Abstract 2,6-Di-O-pentyl-3-O-trifluoroacetylated β-cyclodextrin (DP-TFA-β-CD) as a chiral stationary phase was synthesized and characterized by two-dimensional NMR spectrometry. A chiral fused-silica capillary column prepared by using a mixed stationary phase of DP-TFA-β-CD and OV-7 possesses a high column efficiency of > 4100 plates/m and displays better thermal stability than a column coated with DP-TFA-β-CD alone. Enantiomers such as alcohols, diols, γ-lactones and amines could be separated in relatively short time.