2P-701 : Preparation and Evaluation of D Ionic Liquids Hybrid Macroporous Molecularly Imprinted Monolithic Column for Recognition of Proteins by High Performance Liquid Chromatography

A hybrid maccroporous molecularly imprinted monolithic column was synthesized by free radical polymerization within the confines of a stainless-steel column. In the processes, ionic liquid (1-allyl-3-butylimidazolium Br) and acrylamide were used as dual monomers, ionic liquid (1,2-bis [N-N’-vinyl imidazolium] ethane bis Br) and N,N’-methylenebisacrylamide as dual cross-linking agents, and DES as porogen. Bovine serum albumin and lysozyme, which differ greatly in molecular size, isoelectric point, and charge, were representatively selected for imprinted templates to evaluate recognition property of the green solvents hybrid MIP monolith. Some important factors, such as template-monomer molar ratio, total monomer concentration and crosslinking density, were systematically investigated.