Gas chromatographic determination of aromatic carboxylic acids in the fungus oudemansiella mucida

Abstract A gas chromatographic method was developed for determining trimethylsilyl derivatives of aromatic carboxylic acids in mixtures of biological materials. The separation was performed on 1,5-bis( m -phenoxyphenyl)-1,1,3,3,5,5-hexaphenyltrisiloxane as stationary phase. Its efficiency was compared with that of SE-52. The solute—solvent interactions are discussed in terms of Van der Waals forces and the π-electronic structure of the benzene ring. The chromatograms were quantitatively evaluated by the method of peak area normalization using correction factors. The relative standard deviation of the method was less than 2%. The method was used for analyzing extracts of mycelia and media from cultivations of the basidiomycete Oudemansiella mucida and for investigating the intermediary metabolism of the fungus.