Efeito da adição de diferentes antioxidantes em diluentes de criopreservação sobre a qualidade do sêmen e a produção in vitro de embriões bovinos

The quality of semen samples is extremely important for the success of artificial insemination programs in cattle. Thus, it is essential to maintain the characteristics of spermatozoa through an adequate cryopreservation process. However, cryopreservation processes result in the continuous and excessive formation of reactive oxygen species (EROS), which are harmful to sperm, as it induces apoptosis with a consequent reduction in sperm quality. Thus, cryoprotectants have been added to antioxidant substances such as glutathione and melatonin which aim to minimize the deleterious effects caused by EROS to sperm, resulting in better sperm quality, with a consequent increase in in vitro fertilization rates. Therefore, the present review aims to analyze the effects of the addition of antioxidants glutathione and melatonin in different media of cryopreservation and in vitro production of bovine embryos.