Study on inhibition of genistein on doxorubicin-resistant bladder tumor cells T24

Objective To establish multi-drug resistant bladder (MDR) tumor T24 cell lines and to assess their resistant characteristics.To observe effect of genistein on doxorubicin (ADM) resistant cell lines T24/ADM.Methods Bladder tumor T24 cell line was exposed to ADM in the culture medium for the establishment of drug resistant cell lines:concentrations of ADM was stepwise increased for long exposure.Morphologic studies were performed with optical microscopy.Drug sensitivities were determined by MTT.Results Six months were taken to establish drug resistant cell lines T24/ADM.No obvious morphologic changes were observed between resistant and parental cell. But drug resistances to ADM, 5-Fu,cyclophosphamide and cisplatin were increased,and resistance index were 15.79,4.68,5.53 and 3.81,respectively.Among all groups,there were significant differences.After genistein was used to T24/ADM cells,the IC50 value of genistein was 40 μg/ml.The proliferation cells were induced by genistein at the concentration of 20-100 μg/ml. Conclusion Genistein can inhibit human urinary bladder cancer T24/ADM cell proliferation at some concentration. Key words: Urinary bladder tumor cell line T24;  Multidrug resistance;  Doxorubicin;  Genistein