Study on the HPLC Fingerprint of Chemical Constituents in Ethyl Acetate Parts of SEMEN PLANTAGINIS

[Objective] To establish the fingerprint of chemical constituents of ethyl acetate parts by HPLC,so as to provide a basis for quality control and variety identification of SEMEN PLANTAGINIS.[Method] The chromatographic separation was performed on a C18 column(250 mm×4.6 mm,5 μm) using the mixture of acetonitrile-0.5% acetic acid aqueous solution as mobile phase in gradient elution.The flow rate was 1.0 ml/min,and the detection wavelength was 270 nm.[Result] There were 12 common peaks in the HPLC fingerprint of chemical constituents of ethyl acetate parts of 10 batches of SEMEN PLANTAGINI produced in Jiangxi authentic regions.Fingerprint similarity of ethyl acetate parts of SEMEN PLANTAGINI varied obviously for different varieties.[Conclusion] The HPLC fingerprint method was simple,rapid and feasible in the quality control and varieties identification of SEMEN PLANTAGINI.