The Effect of Dietary Fiber Intake on Systemic Lupus Erythematosus (SLE) Disease in NZB/W Lupus Mice
2020
Dysbiosis in the gut microbiota has been observed in a various autoimmune disease, including SLE, which could
cause a leaky gut, triggering an immune response, and thus worsening autoimmune disease expression. In our current
studies, we hypothesized that increasing dietary fiber would create a healthy microbiota environment in the gut,
leading to decreased leakiness of the gut and to decreased disease expression in and NZB/NZW female lupus-like
mice. NZB/NZW mice were placed on standardized high fiber (HF 30%) or low fiber (LF 0.4%) for 12 weeks
beginning at 20 weeks of age. Mice were assessed as they aged for various parameters of disease including proteinuria
and anti-dsDNA antibody production. Alteration of the microbiota and short chain fatty acid (SCFA) levels were also
assessed. At 36 weeks-of-age, the mice were euthanized, and we assessed occlusion protein expression, splenocyte
profiles, and kidney tissue. We found as the mice aged, their body weights, anti-dsDNA antibody levels, and
proteinuria were not significantly different between the groups. Similarly, there were no significant differences in
SCFA levels. Regarding the microbiota, Chlostridiales bacteria were consistently increased in the HF treated mice
compared to the LF treated mice. Furthermore, as the mice aged, disease progression as assessed by - spleen weight,
immune cell profiles, proteinuria, dsDNA levels, and kidney pathology, was not different between the HF and LF
treated groups. Taken together, these results indicate that in the NZB/W female lupus mouse model, a HF diet may
alter the microbiota but does not influence disease progression.
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