胚龄、NAA浓度、基因型对杂交小麦及其亲本幼胚培养的影响

As an experimental material, XZ1, XZ5 and their parents, uses immature embryos of wheat tissue culture. The effects of age of immature embryos, NAA and different wheat cultivars on the frequency of seedling and growth of the tissue culture from immature embryos of wheat were reported in this paper. The experiment results indicated that the age of immature embryos for its tissue culture was 16d; The optimum concentration of NAA was 0.130 mg/L; The rate of germination of hybrid wheat embryo significantly higher than that of self-wheat embryo; In self-wheat varieties, Fp2 results better cultured seedlings. From all these accounts, we can safely say that the age of immature embryos, concentration of NAA and genotype obviously took regulative effects on the tissue culture from immature embryos of wheat. In practice, the integrating of these effects was riot only helpful to increase the efficiency of the tissue culture from immature embryos of wheat, but also helpful to shorten the breeding period and promote the breeding process.