ANTI -BACTERIAL EVALUATION OF PLANT Nyctanthes arbor-tristis LINN

Nyctanthes arbor-tristis Linn family nyctantheaceae is widely distributed and easily available in the nature, therapeutic use of this plant is detailed in charaka samhitha, Sushrutha samhita and other traditional systems of medicine. Nyctanthes arbor-tristis Linn utilize in the treatment of Seeds are cooling and are beaten into a paste with the juice of Cynodon dactylon and applied to herpatic eruptions.The seeds are cooling and astringent and are useful in vitiated condition of pitta.Seeds are useful in bilious disorders, yield fatty oil used as an illuminant. Oil from seed is used for poisonous bites, bowel complaints, epilepsy and for blackening the hair. One teaspoonful seeds have cooling action especially in bilious disorders .1 Leaves are Antibilious, decoction, laxative, draphoretic, diuretic, cholagogue, mild bitter tonic and expectorant. It is used in fevers, sciatica and rheumatism. 2 Bark are Expectorant .In Malabar the plant is supposed to be alexipharmic and to have the power of removing pains and aches. The drug is also used as hepatoprotective. 3 The dried leaves was subjected to successive extraction using different solvent such as petroleum ether, chloroform, ethyl acetate, and methanol. These solvent extracts were subjected to a Phytochemical evaluation to detect the different chemical principle present i.e. Carbohydrates, Protein, Amino acids, Steroids, Glycosides, Alkaloids, Tannins, and Phenolic compounds and to carry out Chromatographic evaluation to isolate the chemical compounds. The present study was aimed at Chromatographic evaluation of Nyctanthes arbor-tristis Linn leaves. Methanol, Petroleum ether extract of leaves of Nyctanthes arbor-tristis Linn was screened invitro anti-microbial investigations . The antibacterial activity of the various extracts of bark of Nyctanthes arbor - tristis Linn. was evaluated by the filter paper disc method. This method is based on the diffusion of an antibiotic from a filter paper disc through the solified culture media of a petridish used for study. Growth of inoculated micro organism is inhibited entirely in a circular area " Zone", around the filter paper disc containing a solution of the antibiotic and the plant extract. 4