Anti-PD-1 protein monoclonal antibody hybridomas cell, anti- PD-1 monoclonal antibody generated by same and application

The invention relates to the technological field of biology, and discloses an anti-PD-1 protein monoclonal antibody hybridomas cell, an anti- PD-1 monoclonal antibody generated by same and application. The preservation number of the anti-PD-1 protein monoclonal antibody hybridomas cell is CGMCC NO: 11085. The provided hybridomas cell is capable of stably secreting and generating the anti-PD-1 protein monoclonal antibody, and the anti-PD-1 protein monoclonal antibody is specifically combined with PD-1 protein while having no cross reaction with almost 10000 kinds of protein on a protein chip, and thus specificity, accuracy and reliability of PD-1 protein immunodetection are substantially improved, and the anti-PD-1 protein monoclonal antibody is widely applicable to PD-1 detection on various tumor tissue and microenvironment thereof.