A High-throughput Image Correlation Method for Rapid Analysis of Fluorophore Photoblinking and Photobleaching Rates

Simon Sehayek,Yasser Gidi,Viktorija Glembockyte,Hugo B. Brandão,Paul François,Gonzalo Cosa,Paul W. Wiseman

A High-throughput Image Correlation Method for Rapid Analysis of Fluorophore Photoblinking and Photobleaching Rates

2020

Simon Sehayek
Yasser Gidi
Viktorija Glembockyte
Hugo B. Brandão
Paul François
Gonzalo Cosa
Paul W. Wiseman

Super-resolution fluorescence imaging based on localization microscopy requires tuning the photoblinking properties of fluorescent dyes employed. Missing is a rapid way to analyze the blinking rate...

Keywords:

Fluorophore
Throughput
Photobleaching
Biophysics
Biochemistry
Digital image correlation
Biology

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