Abstract 12671: Distinct Metabolic Flow Enables Large-Scale Purification of Pluripotent Stem Cell-Derived Cardiomyocytes

Background Mass production of highly purified cardiomyocytes is a critical bottleneck in realizing heart regenerative therapy. Our recently established non-genetic purification method of cardiomyocytes using mitochondrial dye is efficient but not suitable to produce clinical-scale cardiomyocytes due to the usage of FACS. This study was designed to establish a large-scale purification method for pluripotent stem cell (PSC)-derived cardiomyocytes. Methods and results (1) We first approached this issue by focusing on the possible metabolic differences between cardiomyocytes and embryonic stem cells (ESCs) by transcriptome and metabolome analyses. Transcriptome analysis delineated marked differences in isozyme expression and mRNA levels between metabolic pathways including glycolysis and the TCA cycle. Fluxome analysis using [13C]-labeled glucose revealed that ESCs used glucose mainly for amino acid and nucleotide synthesis, while cardiomyocytes used glucose primarily for ATP production via TCA cycle. (2) Lac...