DETECTION OF MULTI–DRUG RESISTANT SALMONELLA FROM MILK AND MEAT IN BANGLADESH

This study was conducted to investigate the prevalence of Salmonella spp . in milk, chicken meat and beef and to determine the multi-drug resistance (MDR) profile of Salmonella spp . in Mymensingh and Gazipur districts, Bangladesh. A total of 169 samples of milk (n=108), chicken meat (n=51) and beef (n=10) were collected from Bangladesh Agricultural University (BAU) dairy farm, American dairy farm, Gazipur and different  small dairy farms of municipal area during July 2016 to June 2017. Salmonella spp. were isolated on various selective agar media such as: Salmonella-Shigella (SS) agar, Xylose-Lysine Deoxycholate (XLD) agar, Eosine-Methylene Blue (EMB) agar. Identification of Salmonella spp. was done by colony characteristics, Gram staining, biochemical test and Polymerase Chain Reaction (PCR). Multi-drug resistant Salmonella spp. was detected by disc diffusion test using 10 commonly used antibiotics. The overall prevalence of Salmonella spp. in all food samples was 21.89%. A total of 29 (56.86%) chicken meat, 02 (1.85%) milk, and 06 (60%) beef samples were Salmonella spp . positive. Antibiogram study showed that an overall 89.19% of Salmonella spp. was found multi-drug resistant. Specifically 100%, 66.67% and 93.10% of the Salmonella spp . isolates originated from milk, beef and chicken meat respectively were multi-drug resistant. The result of this study suggests that MDR Salmonella spp. is prevalent in the milk and meat which might cause public health hazard if proper hygienic measures are not undertaken at farm and marketing level.